opti-mem reduced serum medium (Thermo Fisher)
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opti-mem reduced serum medium
Opti Mem Reduced Serum Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/opti-mem+reduced+serum+medium/opti+mem+i+reduced+serum+medium/pmc12272859-36-0-5
Average 90 stars, based on 1 article reviews
Opti Mem Reduced Serum Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/opti-mem+reduced+serum+medium/opti+mem+i+reduced+serum+medium/pmc12272859-36-0-5
Average 90 stars, based on 1 article reviews
opti-mem reduced serum medium - by Bioz Stars,
2026-09
90/100 stars
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Plasmid Preparation:Article Title: Compositions and methods for making epigenetic modifications Article Snippet: Plasmid Transfection Transient transfection experiments in N2a cells were performed in 6-well plates using TransIT-LT1 Transfection Reagent (Mirus Bio, 10767-122) and Article Title: Supporting Information for Peptide-based allosteric inhibitor targets TNFR1 conformationally active region and disables receptor-ligand signaling complex Article Snippet: The FRET biosensors were generated by transient transfection of HEK293 cells in 6-well plate with the respective donor-only (0.2 μg) and donor (0.2 μg)/acceptor (1.8 μg) FRET pair DNAs at 1:9 ratio using Lipofectamine 3000 and Article Title: Intrauterine inflammation induced neonatal lung injury via succinic acid-mediated alveolar epithelial E-cadherin downregulation. Article Snippet: 206 For transfection, siRNA targeting SDHB (siSDHB) and scrambled negative control 207 siRNA (siNC) (RIBOBIO) were diluted in Article Title: TNIK-driven regulation of ERK5 transcriptional activity in endothelial cells Article Snippet: ECs were transfected in Article Title: Protocol for high-efficiency generation of iPSCs stably expressing Cas9-EGFP using the selection by essential gene exon knockin method Article Snippet: Article Title: The SDF-1α/MTDH axis inhibits ferroptosis and promotes the formation of anti-VEGF-resistant choroidal neovascularization by facilitating the nuclear translocation of SREBP1 Article Snippet: Then, 20 μM siRNA (siVEGFR2, siSREBP1, siMTDH) was added to OPTI-MEM Reduced Serum Medium (Gibco, USA) and mixed with Article Title: Pathogenic BRCA1 DBD variants exhibit altered DNA binding affinities and susceptibility to menadione Article Snippet: Transient transfection of wild-type and variant plasmids was conducted using Gibco Article Title: RBM8A promotes gastric cancer progression by binding with UPF3B to induce BBC3 mRNA degradation. Article Snippet: The Transfection:Article Title: Compositions and methods for making epigenetic modifications Article Snippet: Plasmid Transfection Transient transfection experiments in N2a cells were performed in 6-well plates using TransIT-LT1 Transfection Reagent (Mirus Bio, 10767-122) and Article Title: Supporting Information for Peptide-based allosteric inhibitor targets TNFR1 conformationally active region and disables receptor-ligand signaling complex Article Snippet: The FRET biosensors were generated by transient transfection of HEK293 cells in 6-well plate with the respective donor-only (0.2 μg) and donor (0.2 μg)/acceptor (1.8 μg) FRET pair DNAs at 1:9 ratio using Lipofectamine 3000 and Article Title: Intrauterine inflammation induced neonatal lung injury via succinic acid-mediated alveolar epithelial E-cadherin downregulation. Article Snippet: 206 For transfection, siRNA targeting SDHB (siSDHB) and scrambled negative control 207 siRNA (siNC) (RIBOBIO) were diluted in Article Title: TNIK-driven regulation of ERK5 transcriptional activity in endothelial cells Article Snippet: ECs were transfected in Article Title: Protocol for high-efficiency generation of iPSCs stably expressing Cas9-EGFP using the selection by essential gene exon knockin method Article Snippet: Article Title: The SDF-1α/MTDH axis inhibits ferroptosis and promotes the formation of anti-VEGF-resistant choroidal neovascularization by facilitating the nuclear translocation of SREBP1 Article Snippet: Then, 20 μM siRNA (siVEGFR2, siSREBP1, siMTDH) was added to OPTI-MEM Reduced Serum Medium (Gibco, USA) and mixed with Article Title: Pathogenic BRCA1 DBD variants exhibit altered DNA binding affinities and susceptibility to menadione Article Snippet: Transient transfection of wild-type and variant plasmids was conducted using Gibco Article Title: RBM8A promotes gastric cancer progression by binding with UPF3B to induce BBC3 mRNA degradation. Article Snippet: The Variant Assay:Article Title: Compositions and methods for making epigenetic modifications Article Snippet: Plasmid Transfection Transient transfection experiments in N2a cells were performed in 6-well plates using TransIT-LT1 Transfection Reagent (Mirus Bio, 10767-122) and Article Title: Supporting Information for Peptide-based allosteric inhibitor targets TNFR1 conformationally active region and disables receptor-ligand signaling complex Article Snippet: The FRET biosensors were generated by transient transfection of HEK293 cells in 6-well plate with the respective donor-only (0.2 μg) and donor (0.2 μg)/acceptor (1.8 μg) FRET pair DNAs at 1:9 ratio using Lipofectamine 3000 and Article Title: Intrauterine inflammation induced neonatal lung injury via succinic acid-mediated alveolar epithelial E-cadherin downregulation. Article Snippet: 206 For transfection, siRNA targeting SDHB (siSDHB) and scrambled negative control 207 siRNA (siNC) (RIBOBIO) were diluted in Article Title: TNIK-driven regulation of ERK5 transcriptional activity in endothelial cells Article Snippet: ECs were transfected in Article Title: Protocol for high-efficiency generation of iPSCs stably expressing Cas9-EGFP using the selection by essential gene exon knockin method Article Snippet: Article Title: The SDF-1α/MTDH axis inhibits ferroptosis and promotes the formation of anti-VEGF-resistant choroidal neovascularization by facilitating the nuclear translocation of SREBP1 Article Snippet: Then, 20 μM siRNA (siVEGFR2, siSREBP1, siMTDH) was added to OPTI-MEM Reduced Serum Medium (Gibco, USA) and mixed with Article Title: Pathogenic BRCA1 DBD variants exhibit altered DNA binding affinities and susceptibility to menadione Article Snippet: Transient transfection of wild-type and variant plasmids was conducted using Gibco Article Title: RBM8A promotes gastric cancer progression by binding with UPF3B to induce BBC3 mRNA degradation. Article Snippet: The Generated:Article Title: Compositions and methods for making epigenetic modifications Article Snippet: Plasmid Transfection Transient transfection experiments in N2a cells were performed in 6-well plates using TransIT-LT1 Transfection Reagent (Mirus Bio, 10767-122) and Article Title: Supporting Information for Peptide-based allosteric inhibitor targets TNFR1 conformationally active region and disables receptor-ligand signaling complex Article Snippet: The FRET biosensors were generated by transient transfection of HEK293 cells in 6-well plate with the respective donor-only (0.2 μg) and donor (0.2 μg)/acceptor (1.8 μg) FRET pair DNAs at 1:9 ratio using Lipofectamine 3000 and Article Title: Intrauterine inflammation induced neonatal lung injury via succinic acid-mediated alveolar epithelial E-cadherin downregulation. Article Snippet: 206 For transfection, siRNA targeting SDHB (siSDHB) and scrambled negative control 207 siRNA (siNC) (RIBOBIO) were diluted in Article Title: TNIK-driven regulation of ERK5 transcriptional activity in endothelial cells Article Snippet: ECs were transfected in Article Title: Protocol for high-efficiency generation of iPSCs stably expressing Cas9-EGFP using the selection by essential gene exon knockin method Article Snippet: Article Title: The SDF-1α/MTDH axis inhibits ferroptosis and promotes the formation of anti-VEGF-resistant choroidal neovascularization by facilitating the nuclear translocation of SREBP1 Article Snippet: Then, 20 μM siRNA (siVEGFR2, siSREBP1, siMTDH) was added to OPTI-MEM Reduced Serum Medium (Gibco, USA) and mixed with Article Title: Pathogenic BRCA1 DBD variants exhibit altered DNA binding affinities and susceptibility to menadione Article Snippet: Transient transfection of wild-type and variant plasmids was conducted using Gibco Article Title: RBM8A promotes gastric cancer progression by binding with UPF3B to induce BBC3 mRNA degradation. Article Snippet: The Negative Control:Article Title: Compositions and methods for making epigenetic modifications Article Snippet: Plasmid Transfection Transient transfection experiments in N2a cells were performed in 6-well plates using TransIT-LT1 Transfection Reagent (Mirus Bio, 10767-122) and Article Title: Supporting Information for Peptide-based allosteric inhibitor targets TNFR1 conformationally active region and disables receptor-ligand signaling complex Article Snippet: The FRET biosensors were generated by transient transfection of HEK293 cells in 6-well plate with the respective donor-only (0.2 μg) and donor (0.2 μg)/acceptor (1.8 μg) FRET pair DNAs at 1:9 ratio using Lipofectamine 3000 and Article Title: Intrauterine inflammation induced neonatal lung injury via succinic acid-mediated alveolar epithelial E-cadherin downregulation. Article Snippet: 206 For transfection, siRNA targeting SDHB (siSDHB) and scrambled negative control 207 siRNA (siNC) (RIBOBIO) were diluted in Article Title: TNIK-driven regulation of ERK5 transcriptional activity in endothelial cells Article Snippet: ECs were transfected in Article Title: Protocol for high-efficiency generation of iPSCs stably expressing Cas9-EGFP using the selection by essential gene exon knockin method Article Snippet: Article Title: The SDF-1α/MTDH axis inhibits ferroptosis and promotes the formation of anti-VEGF-resistant choroidal neovascularization by facilitating the nuclear translocation of SREBP1 Article Snippet: Then, 20 μM siRNA (siVEGFR2, siSREBP1, siMTDH) was added to OPTI-MEM Reduced Serum Medium (Gibco, USA) and mixed with Article Title: Pathogenic BRCA1 DBD variants exhibit altered DNA binding affinities and susceptibility to menadione Article Snippet: Transient transfection of wild-type and variant plasmids was conducted using Gibco Article Title: RBM8A promotes gastric cancer progression by binding with UPF3B to induce BBC3 mRNA degradation. Article Snippet: The Incubation:Article Title: Compositions and methods for making epigenetic modifications Article Snippet: Plasmid Transfection Transient transfection experiments in N2a cells were performed in 6-well plates using TransIT-LT1 Transfection Reagent (Mirus Bio, 10767-122) and Article Title: Supporting Information for Peptide-based allosteric inhibitor targets TNFR1 conformationally active region and disables receptor-ligand signaling complex Article Snippet: The FRET biosensors were generated by transient transfection of HEK293 cells in 6-well plate with the respective donor-only (0.2 μg) and donor (0.2 μg)/acceptor (1.8 μg) FRET pair DNAs at 1:9 ratio using Lipofectamine 3000 and Article Title: Intrauterine inflammation induced neonatal lung injury via succinic acid-mediated alveolar epithelial E-cadherin downregulation. Article Snippet: 206 For transfection, siRNA targeting SDHB (siSDHB) and scrambled negative control 207 siRNA (siNC) (RIBOBIO) were diluted in Article Title: TNIK-driven regulation of ERK5 transcriptional activity in endothelial cells Article Snippet: ECs were transfected in Article Title: Protocol for high-efficiency generation of iPSCs stably expressing Cas9-EGFP using the selection by essential gene exon knockin method Article Snippet: Article Title: The SDF-1α/MTDH axis inhibits ferroptosis and promotes the formation of anti-VEGF-resistant choroidal neovascularization by facilitating the nuclear translocation of SREBP1 Article Snippet: Then, 20 μM siRNA (siVEGFR2, siSREBP1, siMTDH) was added to OPTI-MEM Reduced Serum Medium (Gibco, USA) and mixed with Article Title: Pathogenic BRCA1 DBD variants exhibit altered DNA binding affinities and susceptibility to menadione Article Snippet: Transient transfection of wild-type and variant plasmids was conducted using Gibco Article Title: RBM8A promotes gastric cancer progression by binding with UPF3B to induce BBC3 mRNA degradation. Article Snippet: The Concentration Assay:Article Title: Compositions and methods for making epigenetic modifications Article Snippet: Plasmid Transfection Transient transfection experiments in N2a cells were performed in 6-well plates using TransIT-LT1 Transfection Reagent (Mirus Bio, 10767-122) and Article Title: Supporting Information for Peptide-based allosteric inhibitor targets TNFR1 conformationally active region and disables receptor-ligand signaling complex Article Snippet: The FRET biosensors were generated by transient transfection of HEK293 cells in 6-well plate with the respective donor-only (0.2 μg) and donor (0.2 μg)/acceptor (1.8 μg) FRET pair DNAs at 1:9 ratio using Lipofectamine 3000 and Article Title: Intrauterine inflammation induced neonatal lung injury via succinic acid-mediated alveolar epithelial E-cadherin downregulation. Article Snippet: 206 For transfection, siRNA targeting SDHB (siSDHB) and scrambled negative control 207 siRNA (siNC) (RIBOBIO) were diluted in Article Title: TNIK-driven regulation of ERK5 transcriptional activity in endothelial cells Article Snippet: ECs were transfected in Article Title: Protocol for high-efficiency generation of iPSCs stably expressing Cas9-EGFP using the selection by essential gene exon knockin method Article Snippet: Article Title: The SDF-1α/MTDH axis inhibits ferroptosis and promotes the formation of anti-VEGF-resistant choroidal neovascularization by facilitating the nuclear translocation of SREBP1 Article Snippet: Then, 20 μM siRNA (siVEGFR2, siSREBP1, siMTDH) was added to OPTI-MEM Reduced Serum Medium (Gibco, USA) and mixed with Article Title: Pathogenic BRCA1 DBD variants exhibit altered DNA binding affinities and susceptibility to menadione Article Snippet: Transient transfection of wild-type and variant plasmids was conducted using Gibco Article Title: RBM8A promotes gastric cancer progression by binding with UPF3B to induce BBC3 mRNA degradation. Article Snippet: The Over Expression:Article Title: Compositions and methods for making epigenetic modifications Article Snippet: Plasmid Transfection Transient transfection experiments in N2a cells were performed in 6-well plates using TransIT-LT1 Transfection Reagent (Mirus Bio, 10767-122) and Article Title: Supporting Information for Peptide-based allosteric inhibitor targets TNFR1 conformationally active region and disables receptor-ligand signaling complex Article Snippet: The FRET biosensors were generated by transient transfection of HEK293 cells in 6-well plate with the respective donor-only (0.2 μg) and donor (0.2 μg)/acceptor (1.8 μg) FRET pair DNAs at 1:9 ratio using Lipofectamine 3000 and Article Title: Intrauterine inflammation induced neonatal lung injury via succinic acid-mediated alveolar epithelial E-cadherin downregulation. Article Snippet: 206 For transfection, siRNA targeting SDHB (siSDHB) and scrambled negative control 207 siRNA (siNC) (RIBOBIO) were diluted in Article Title: TNIK-driven regulation of ERK5 transcriptional activity in endothelial cells Article Snippet: ECs were transfected in Article Title: Protocol for high-efficiency generation of iPSCs stably expressing Cas9-EGFP using the selection by essential gene exon knockin method Article Snippet: Article Title: The SDF-1α/MTDH axis inhibits ferroptosis and promotes the formation of anti-VEGF-resistant choroidal neovascularization by facilitating the nuclear translocation of SREBP1 Article Snippet: Then, 20 μM siRNA (siVEGFR2, siSREBP1, siMTDH) was added to OPTI-MEM Reduced Serum Medium (Gibco, USA) and mixed with Article Title: Pathogenic BRCA1 DBD variants exhibit altered DNA binding affinities and susceptibility to menadione Article Snippet: Transient transfection of wild-type and variant plasmids was conducted using Gibco Article Title: RBM8A promotes gastric cancer progression by binding with UPF3B to induce BBC3 mRNA degradation. Article Snippet: The Expressing:Article Title: Compositions and methods for making epigenetic modifications Article Snippet: Plasmid Transfection Transient transfection experiments in N2a cells were performed in 6-well plates using TransIT-LT1 Transfection Reagent (Mirus Bio, 10767-122) and Article Title: Supporting Information for Peptide-based allosteric inhibitor targets TNFR1 conformationally active region and disables receptor-ligand signaling complex Article Snippet: The FRET biosensors were generated by transient transfection of HEK293 cells in 6-well plate with the respective donor-only (0.2 μg) and donor (0.2 μg)/acceptor (1.8 μg) FRET pair DNAs at 1:9 ratio using Lipofectamine 3000 and Article Title: Intrauterine inflammation induced neonatal lung injury via succinic acid-mediated alveolar epithelial E-cadherin downregulation. Article Snippet: 206 For transfection, siRNA targeting SDHB (siSDHB) and scrambled negative control 207 siRNA (siNC) (RIBOBIO) were diluted in Article Title: TNIK-driven regulation of ERK5 transcriptional activity in endothelial cells Article Snippet: ECs were transfected in Article Title: Protocol for high-efficiency generation of iPSCs stably expressing Cas9-EGFP using the selection by essential gene exon knockin method Article Snippet: Article Title: The SDF-1α/MTDH axis inhibits ferroptosis and promotes the formation of anti-VEGF-resistant choroidal neovascularization by facilitating the nuclear translocation of SREBP1 Article Snippet: Then, 20 μM siRNA (siVEGFR2, siSREBP1, siMTDH) was added to OPTI-MEM Reduced Serum Medium (Gibco, USA) and mixed with Article Title: Pathogenic BRCA1 DBD variants exhibit altered DNA binding affinities and susceptibility to menadione Article Snippet: Transient transfection of wild-type and variant plasmids was conducted using Gibco Article Title: RBM8A promotes gastric cancer progression by binding with UPF3B to induce BBC3 mRNA degradation. Article Snippet: The |